17 July 2020
on course website
Organoid Technology and Applications
This course will allow you to learn basic and advanced culturing techniques of organoid stem cell cultures. We will focus on the development, background and most recent high impact science with organoid technology and its applications in research, diagnostics and personalized medicine. This course is comprised of meeting the experts, demonstrations and hands-on bench training. Thereby the use of human intestinal organoids in cystic fibrosis research (FIS assay) is highlighted as an example of a highly efficient application of this novel technology. At the end of the training participants will have basic knowledge of intestinal organoids and their applications.
Since the first successful creation of an intestinal organoid with microvilli structures in 2009 in the Hans Clevers lab in Utrecht, the development of organoid research has been advancing. Extensive research work has improved the research methodology and extended the range of organoids, which can now be produced in the laboratory. To date, liver, brain, pancreas, lung, breast, prostate, intestine, and even tumor tissue organoids have been created. As they are similar to their original organs, organoids hold great promise for use in medical research and the development of new treatments.
In this intensive 5-day course, coordinated by the Utrecht Platform for Organoid Technology (U-PORT), top scientists in the organoid research field will give lectures about their particular fields of research comprising organoids of different types of tissues and applying these organoids in medical research (including CRISPR/Cas9 editing of organoids, drug screening and regenerative medicine purposes). In the practical experiments, participants will be introduced to the basics of working with organoid technology and will also directly apply organoid technology using a functional assay in cystic fibrosis research.
At the end of the training participants will have basic knowledge of intestinal organoids and their applications and are able to culture human intestinal organoids as well as to understand how to apply organoid technology in their own research.
Prof. dr. J.M. Beekman; Prof. dr. O.W. Kranenburg & Prof. dr. M.M. Maurice
Technicians, PhD, Postdocs. Master students are welcome, but we expect basic practical lab skills.
This course aims to teach the participants:
- to learn the basics about the most available tissue types of organoid technology (intestine/ liver/ kidney/ lung/ cancer);
- to learn the theory and basics of intestinal organoid technology and culture (isolation, regular culture, storage/biobanking);
- to be able to efficiently bring this into practice in the reserach lab;
- to apply this in participants' own research area.
EUR 1500: Course + course materials + lunch
EUR 200: Housing fee (optional)
on course website