Extracellular Vesicles in Health and Disease

EVs have been shown to be released from all three cellular domains of life. In recent years, EVs have been recognized to play an essential role in health and disease by trafficking bioactive molecules between cells. This way, EVs are important in disease progression since both diseased cells and pathogens release EVs. This also suggests that EVs can be used as diagnostic markers for such conditions. The therapeutic potential of EVs ('native form' or modified as drug delivery systems) and their use as vaccines are also currently being explored, all underpinning the interdisciplinary nature of EV research.

In this course, we will cover basic aspects of EVs, which will include their nomenclature, biogenesis, release and uptake, as well as EV cargos. Different isolation methods will be introduced, such as ultracentrifugation, size exclusion chromatography and precipitation techniques, and their pros and cons will be discussed. Different methods to identify, characterize and enumerate EVs will be presented and discussed as well as how to explore their content. As it is not a trivial task to work with EVs, we will discuss critical considerations during the collection and isolation of EVs from various sample types, including non-model organisms. We will then discuss how to explore EV function in vitro and in vivo and, lastly the diagnostic and therapeutic potential of EVs (e.g. as drug delivery systems, vaccines).

Course leader

Peter Lindberg Nejsum

Target group

Master's level

Fee info

EUR 380: EU/EEA citizens
EUR 1313: NON-EU/EEA citizens

Scholarships

No scholarships available

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